Gene expression


By demonstration and active participation in training, trainings aim to ease up the incorporation of modern innovative biomedicine and biotechnology methods into the on-going research projects, share experience, and transfer critical know-how to increase the research capacities of Latvian and Ukrainian researchers of all levels.

Training format

The training was organized as presentations of the methods in the form of a conference, and a series of experimental/wet trainings on several modern research techniques used to detect gene/protein expression, namely: quantitative real-time polymerase chain reaction; single cell gel electrophoresis assay; fluorescence activated cell sorting; and immunohistochemistry.

Content of the wet labs

Quantitative real-time PCR (q-PCR): Twenty six people were trained in two groups, due to the high interest from public. Teachers: Larysa Kovalevska, PhD (IEPOR of NASU, UA) and Muhammad Mushtaq, PhD student (MTC, KI, SE).

Fluorescence activated cell sorting (FACS): Twenty one persons have signed for this wet lab, therefore two rounds of training were performed. Teachers: Yulia Shvets, PhD (Taras Shevchenko National University of Kyiv, UA) and Daria Storchay, PhD student (IEPOR of NASU, UA).

Method of immunohistochemistry: Due to the high number of participants (20 individuals), method was trained in two groups, 10 people each. Teachers: Lilia Gumenyuk, PhD and Natalia Iurchenko (both from IEPOR of NASU, UA).

Single Cell Gel Electrophoresis assay: Training was performed for 15 persons. Teachers: Olga Bryeyeva, PhD student and Dmytro Demash, PhD (both from IEPOR of NASU, UA).